解剖学和形态学
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细胞和组织工程学
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牙科,口腔外科和医学
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abstract::Sulfated O-linked oligosaccharides from the sea urchin egg receptor have been shown to bind to acrosome-reacted sperm and to inhibit fertilization in a competitive bioassay. However, the inhibitory activity of these isolated chains was much lower than that of a recombinant protein representing a portion of the extrace...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/6.1.59
更新日期:1996-01-01 00:00:00
abstract::CAMPATH-1H (where CAMPATH is a trade mark of Wellcome group companies), a humanized IgG antibody used in the therapy of lymphoma, leukaemia and rheumatoid arthritis, has been expressed in Chinese hamster ovary, Y0 myeloma and NS0 myeloma cell lines. These engineered cell lines were grown under different culture condit...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.8.813
更新日期:1995-12-01 00:00:00
abstract::The biosynthesis of the Lewis X determinant (Gal beta 1-4 [Fuc alpha 1-3]GlcNAc beta-) in three strains of Helicobacter pylori has been investigated. Strains UA 861, UA 802 and UA 1182 contain alpha 1,3 fucosyltransferase and beta 1,4 galactosyltransferase activities that synthesize the Lewis X structure by the transf...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.7.683
更新日期:1995-10-01 00:00:00
abstract::Trypanosoma cruzi expresses a unique trans-sialidase that is responsible for the transfer of sialic acid from host glycoproteins and glycolipids to mucin-like glycoprotein acceptors on the parasite surface. The enzyme and the sialic acid acceptors are present in the mammalian forms of the parasite and in the parasite ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.6.625
更新日期:1995-09-01 00:00:00
abstract::To investigate the developmental role of complex N-linked oligosaccharides, we previously inactivated the mouse Mgat1 gene which encodes UDP-N-acetylglucosamine: alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I (GlcNAc-TI). Mgat1-null embryos developed morphogenic abnormalities by embryonic day (E) 9.5 a...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.5.535
更新日期:1995-07-01 00:00:00
abstract::Lymphocytic beta 1,4-galactosyltransferase (beta 1,4-GalTase, EC 2.4.1.38) activity was measured in B cells using a neoglyco-protein, N-acetylglucosamine-phenylisothiocyanate-bovine serum albumin (GlcNAc-pITC-BSA), as an acceptor substrate in a novel enzyme-linked immunosorbent assay (ELISA)-based method. This assay p...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.4.365
更新日期:1995-06-01 00:00:00
abstract::We have characterized a glycosphingolipid (GSL) receptor for Staphylococcus enterotoxin-B (SEB) in cultured human kidney proximal tubular (PT) cells. Solid-phase binding of [125I]SEB to the GSL receptor was concentration dependent and was not displaceable by two structurally related toxins, such as staphylococcal ente...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.3.327
更新日期:1995-05-01 00:00:00
abstract::Protozoan parasites of the genus Leishmania have a glycoconjugate surface coat (the glycocalyx) that acts as the interface between the parasite and its external environment. The principal components of the glycocalyx, the lipophosphoglycans and the glycoinositolphospholipids, have a variety of functions that facilitat...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/5.2.161
更新日期:1995-03-01 00:00:00
abstract::Oral treatment of mice, cutaneously infected with herpes simplex virus type 1 (HSV-1) (strain SC16), with the alpha-glucosidase 1 inhibitor 6-O-butanoyl castanospermine (MDL 28,574) produced a significant delay in lesion development and reduced the amount of virus recovered from the brain. Virus load in the brains of ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.2.249
更新日期:1995-03-01 00:00:00
abstract::Based on chemical analysis, we have previously concluded that the biologically important lipoarabinomannan (LAM) and lipomannan (LM) from Mycobacterium are multiglycosylated forms of the phosphatidylinositol mannosides (PIMs), the characteristic cell envelope mannophosphoinositides of mycobacteria. Using definitive an...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.1.117
更新日期:1995-02-01 00:00:00
abstract::The deletion of the protein mannosyltransferase 1 gene (PMT1) of Saccharomyces cerevisiae results in viable cells. O-Mannosylation of proteins is reduced to about half of the value in comparison to wild-type cells. In order to distinguish between the the PMT1 gene product (= Pmt1p) and residual transferase activity, a...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.1.77
更新日期:1995-02-01 00:00:00
abstract::Human colon cancer is associated with antigenic and structural changes in mucin-type carbohydrate chains (O-glycans). To elucidate the control of the biosynthesis of these O-glycans is colon cancer, we have studied glycosyltransferase and sulphotransferase activities involved in the assembly of elongated O-glycan stru...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.6.873
更新日期:1994-12-01 00:00:00
abstract::Cytotrophoblasts are the specialized epithelial cells of the placenta. During the first trimester, a subpopulation of chorionic villus cytotrophoblasts differentiates along an invasive pathway and penetrates the maternal endometrium, decidua and spiral arterioles. Cytotrophoblast invasiveness declines rapidly during t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.5.567
更新日期:1994-10-01 00:00:00
abstract::We recently described a novel fluorescent compound, 2-amino,6-amidobiotinyl-pyridine (BAP), that allows the tagging of oligosaccharides, their fractionation by reversed-phase HPLC with picomole scale detection, and the formation of functional neoglycoprotein equivalents with (strept) avidin for the detection of recept...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.5.653
更新日期:1994-10-01 00:00:00
abstract::In this study, a sensitive, straightforward technique is developed for the analysis of glycoprotein O-linked oligosaccharides. Specifically, O-linked oligosaccharides of granulocyte colony-stimulating factor (G-CSF) are analysed by separating charged glycoforms using isoelectric focusing, electroblotting to polyvinyli...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.4.459
更新日期:1994-08-01 00:00:00
abstract::A protocol is described for uniform 13C labelling of terminal galactose residues of the glycan chains of glycoproteins, using an enzymatic method which does not perturb the protein. The technique is illustrated by application to the biantennary N-linked glycan chains attached at Asn 297 of immunoglobulin G (IgG). Isot...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.4.485
更新日期:1994-08-01 00:00:00
abstract::Influenza C virus spike glycoprotein HEF specifically recognizes glycoconjugates containing 9-O-acetyl-N-acetylneuraminic acid. The same protein also contains an esterase activity. Taking advantage of these two properties, influenza C virus was used as a very sensitive probe for the detection of traces of 9-O-acetyl-N...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.3.343
更新日期:1994-06-01 00:00:00
abstract::Recombinant human erythropoietin (rHuEPO) is used abundantly in the clinic to stimulate red blood cell growth in anaemic patients. The efficacy of the drug depends strongly on the extent of sialylation of its carbohydrate moiety. Prompted by conflicting literature reports on the issue, we reinvestigated the structures...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.2.227
更新日期:1994-04-01 00:00:00
abstract::The human anticoagulant factor, Protein C, is a plasma glycoprotein that has reported anti-ischaemic and anti-inflammatory properties. To explore potential mechanisms for these reported activities, we examined the effect of Protein C on the process of cell adhesion to vascular endothelial cells, which plays a critical...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.2.221
更新日期:1994-04-01 00:00:00
abstract::Mac-2 is a member of the S-(soluble) lectin family. Its identification and isolation from a wide variety of cell types and tissues suggest a diversity of roles in various biological systems. The key points to be made are that Mac-2, and the S-lectins in general, by virtue of their recognition of a variety of carbohydr...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/4.1.5
更新日期:1994-02-01 00:00:00
abstract::Phytohaemagglutinin (PHA) is the major lectin present in the seeds of the common bean, Phaseolus vulgaris, and PHA-L is the leucocyte-agglutinating form of this lectin. This tetrameric glycoprotein accumulates in the vacuoles of storage parenchyma cells. Based on amino acid sequence comparisons of legume lectins and t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.6.581
更新日期:1993-12-01 00:00:00
abstract::Proliferation of exponentially growing breast cancer cells (line Hs578T) was blocked specifically in G1 by 3-hydroxy-3-methylglutaryl Coenzyme A (HMG CoA) reductase inhibition, as well as by inhibition of N-linked glycosylation. As a consequence of these inhibitory conditions, the cells were synchronized in the G1 sta...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.5.475
更新日期:1993-10-01 00:00:00
abstract::In the yeast Saccharomyces cerevisiae, the alg2 mutation causes temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol (Jackson et al., Arch. Biochem. Biophys., 272, 203-209, 1989; Huffaker and Robbins, Proc. Natl. Acad. Sci. USA, 80, 7466-7470, 1983). A gene havi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.4.357
更新日期:1993-08-01 00:00:00
abstract::The N-glycans have been removed by peptide-N-glycosidase F (PNGase F) from purified human non-secretory RNases derived from kidney, liver and spleen. The spleen RNase was purified by two procedures, one of which did not include the usual acid treatment step (0.25 M H2SO4, 45 min, 4 degrees C), to determine if acid tre...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.3.249
更新日期:1993-06-01 00:00:00
abstract::We investigated the localization of major gangliosides in adult rat brain by an immunofluorescence technique with mouse monoclonal antibodies (MAbs). Five MAbs (GMB16, GMR17, GGR12, GMR5 and GMR13) that specifically recognize gangliosides GM1, GD1a, GD1b, GT1b and GQ1b, respectively, were used. We have found that ther...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.2.137
更新日期:1993-04-01 00:00:00
abstract::The concanavalin A staining of cellular glycoproteins and the direct analysis of their sugar chains released by hydrazinolysis revealed that the processing of N-linked sugar chains of some glycoproteins is suppressed by exposure of mouse monocytoid cells P388D1 to dimethyl sulphoxide, which can induce Fc receptor-medi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.1.15
更新日期:1993-02-01 00:00:00
abstract::Lipid-A represents the ubiquitous, covalently bound hydrophobic component of bacterial lipopolysaccharides (endotoxins). Lipid-As isolated and characterized from rhizobial species have large variations in their backbone sugars, as well as in their hydroxy fatty acid substituents. The sugar backbones consist of either ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/2.6.535
更新日期:1992-12-01 00:00:00
abstract::Sulphated N-linked carbohydrate chains isolated from recombinant human tissue plasminogen activator expressed in mouse epithelial (C127) cells were analysed as oligosaccharide alditols by methylation analysis, liquid secondary ion mass spectrometry, and one- and two-dimensional 1H-NMR spectroscopy. The results demonst...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/2.5.411
更新日期:1992-10-01 00:00:00
abstract::L-Selectin is a lectin-like receptor on lymphocytes which mediates their attachment to high endothelial venules (HEV) within lymph nodes. Previous work has identified HEV-associated endothelial ligands for L-selectin as sialylated, fucosylated and sulphated glycoproteins of approximately 50 kDa and approximately 90 kD...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/2.4.373
更新日期:1992-08-01 00:00:00
abstract::Knowledge of the identity, synthesis and secretion of beta-galactoside-binding lectins by leukocytes is of importance because lactosaminoglycans present at the leukocyte cell surface may be physiologically significant lectin receptors that could mediate autocrine or paracrine functions and/or cell adhesion. This paper...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/2.4.285
更新日期:1992-08-01 00:00:00
abstract::Lutrophin (LH) consists of an array of isoforms with different charges and bioactivities. This study was undertaken to clarify specifically how oligosaccharides of alpha and beta subunits contribute to LH isoform charges. Porcine LH (pLH) was separated into four isoforms by isoelectric focusing (IEF), followed by subu...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/2.3.225
更新日期:1992-06-01 00:00:00
abstract::Saccharomyces cerevisiae Man9-alpha-mannosidase, responsible for trimming Man9GlcNAc2 in the endoplasmic reticulum to Man8GlcNAc2, the substrate for oligosaccharide elongation, has been purified to homogeneity from stabilized microsomal membranes without employing autolytic digestion. The activity was solubilized by t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/1.6.605
更新日期:1991-12-01 00:00:00
abstract::The substrate specificity of the alpha-D-mannosidases of rat liver lysosome and cytosol was examined using oligosaccharides of the oligomannosidic type. The hydrolysis products were characterized by 400 MHz 1H-NMR spectroscopy. Both catabolic pathways occur in ordered ways, but are quite different. In fact, the lysoso...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/1.5.487
更新日期:1991-11-01 00:00:00
abstract::Glycopeptides representing individual N-glycosylation sites of the heterodimeric glycoprotein hormone human chorionic gonadotrophin (hCG) were obtained from subunits hCG alpha (N-glycosylated at Asn-52 and Asn-78) and hCG beta (N-glycosylated at Asn-13 and Asn-30) by digestion with trypsin and chymotrypsin, respective...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/1.4.393
更新日期:1991-09-01 00:00:00
abstract::In this report we describe a system capable of resolving all of the known unsaturated disaccharides derived from the chondroitin sulphates, dermatan sulphate and hyaluronic acid by chondroitinase digestion. This system is superior to others in that the non-sulphated and mono-, di- and tri-sulphated disaccharides can b...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/1.3.271
更新日期:1991-06-01 00:00:00
abstract::We have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the pu...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/1.2.173
更新日期:1991-03-01 00:00:00